Purification of rat testicular microsomal 17-ketosteroid reductase. Evidence that 17-ketosteroid reductase and 17 beta-hydroxysteroid dehydrogenase are distinct enzymes.
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چکیده
منابع مشابه
Characteristics of a 17-ketosteroid reaction.
An improved method for the determination of 17-ketosteroids is described, which involves an increase in sensitivity by the simple expedient of upsetting the equilibrium of the reaction in removing the products of reaction by precipitating the chromophore formed. This precipitation system appears to achieve about 1.6 times the color sensitivity for the same reaction carried out as a solution pro...
متن کاملEffect of Cortisone on 17-Ketosteroid Excretion *
In 1952 cortisone therapy was initiated in two patients with far advanced prostatic carcinoma.2 As part of the study of those patients, determinations were made of urinary 17-ketosteroid excretion before and during therapy with cortisone. Rather large increases in excretion of 17-ketosteroids were noted during the administration of high doses of cortisone in both patients. This unexpected findi...
متن کاملRegulation of the gene for estrogenic 17-ketosteroid reductase lying on chromosome 17cen----q25.
17-Ketosteroid reductase (17KSR), also known as 17 beta-hydroxysteroid dehydrogenase, catalyzes the reversible interconversion of estradiol to estrone and of androstenedione to testosterone. Using a recently cloned human placental 17KSR cDNA, we show that the 1.4-kilobase mRNA for this enzyme is detected only in tissues producing estrogens, and a 2.4-kilobase mRNA is detected in some estrogenic...
متن کامل17beta-hydroxysteroid dehydrogenase type 7--an ancient 3-ketosteroid reductase of cholesterogenesis.
17beta-hydroxysteroid dehydrogenase type 7 (17beta-HSD7) is a novel estrogenic hydroxysteroid dehydrogenase from mammals. We modeled the three-dimensional structure of human 17beta-HSD7, analyzed the phylogeny of 17beta-HSD7 homologues and determined its expression pattern by in silico Northern blotting. Predominant expression is found not only in reproductive tissues (breast, ovary, placenta) ...
متن کاملA method for the quantitative analysis of 17-ketosteroid mixtures.
Neher and Wettstein (1) have recently published the details of a paper chromatographic method for the separation of weakly polar steroids, with a heptane-phenyl Cellosolve solvent system. Savard (2) has described the separation of 17-ketosteroids on paper with ligroin as the mobile phase and propylene glycol as the stationary phase. Starting from the basic work of these authors, a method has be...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)70665-6